Fluorescent microscope system to track a particular region of C. elegans

Mitsunori Maru, Yasunobu Igarashi, Shogo Arai, Koichi Hashimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

C. elegans has been widely studied for understanding the basic mechanisms of nervous system function. In order to examine neural activity in C. elegans, it is necessary to measure a Ca2+ concentration in a neuron. Observers acquire fluorescence images of fluorescent dyes introduced in neurons. Then they can evaluate intensity of the fluorescence images which respond to changes in the Ca2+ concentration. Thus neural activity is examined by the fluorescence images of the neuron. However, observing the specified neuron in C. elegans for a long time is very difficult since a head of C. elegans moves quickly. To solve this problem, we develop a microscope system which can track a particular region of C. elegans and monitor fluorescence emitted by fluorescent protein in C. elegans. In experimental results, we show that the microscope system can track the head region of moving C. elegans and monitor fluorescence emitted by a chemosensory neuron ASER at 20x magnification.

Original languageEnglish
Title of host publication2010 IEEE/SICE International Symposium on System Integration
Subtitle of host publicationSI International 2010 - The 3rd Symposium on System Integration, SII 2010, Proceedings
Pages347-352
Number of pages6
DOIs
Publication statusPublished - 2010 Dec 1
Event3rd International Symposium on System Integration, SII 2010 - Sendai, Japan
Duration: 2010 Dec 212010 Dec 22

Publication series

Name2010 IEEE/SICE International Symposium on System Integration: SI International 2010 - The 3rd Symposium on System Integration, SII 2010, Proceedings

Other

Other3rd International Symposium on System Integration, SII 2010
CountryJapan
CitySendai
Period10/12/2110/12/22

ASJC Scopus subject areas

  • Control and Systems Engineering

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    Maru, M., Igarashi, Y., Arai, S., & Hashimoto, K. (2010). Fluorescent microscope system to track a particular region of C. elegans. In 2010 IEEE/SICE International Symposium on System Integration: SI International 2010 - The 3rd Symposium on System Integration, SII 2010, Proceedings (pp. 347-352). [5708350] (2010 IEEE/SICE International Symposium on System Integration: SI International 2010 - The 3rd Symposium on System Integration, SII 2010, Proceedings). https://doi.org/10.1109/SII.2010.5708350