Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons

Yasuo Oyama, Akemi Hayashi, Toshiko Ueha, Lumi Chikahisa, Katsutoshi Furukawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidazed to be fluoresent by cellular oxidants. Of the organic Ca2+ antagonist used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 μM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.

Original languageEnglish
Pages (from-to)172-175
Number of pages4
JournalBrain research
Volume610
Issue number1
DOIs
Publication statusPublished - 1993 Apr 30

Keywords

  • 2',7'-Dichlorofluorescin diacetate
  • Brain neuron
  • Ca antagonist
  • Ischmic brain damage
  • Oxidative metabolism

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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