TY - JOUR
T1 - Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons
AU - Oyama, Yasuo
AU - Hayashi, Akemi
AU - Ueha, Toshiko
AU - Chikahisa, Lumi
AU - Furukawa, Katsutoshi
N1 - Funding Information:
This study was partly supported by the research grants from the Ciba-Geigy Foundation for Promotion of Science (Takarazuka, Japan) and the Nippon Life Insurance Foundation (Osaka, Japan).
PY - 1993/4/30
Y1 - 1993/4/30
N2 - Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidazed to be fluoresent by cellular oxidants. Of the organic Ca2+ antagonist used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 μM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.
AB - Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidazed to be fluoresent by cellular oxidants. Of the organic Ca2+ antagonist used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 μM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.
KW - 2',7'-Dichlorofluorescin diacetate
KW - Brain neuron
KW - Ca antagonist
KW - Ischmic brain damage
KW - Oxidative metabolism
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U2 - 10.1016/0006-8993(93)91234-J
DO - 10.1016/0006-8993(93)91234-J
M3 - Article
C2 - 8518926
AN - SCOPUS:0027294857
VL - 610
SP - 172
EP - 175
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -