Fluorescent estimation on cytotoxicity of methylmercury in dissociated rat cerebellar neurons: Its comparison with ionomycin

Eisuke Okazaki, Yasuo Oyama, Lumi Chikahisa, Takayuki Nagano, Norihiro Katayama, Mineshi Sakamoto

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

To study the cellular basis of the neurotoxicity of methylmercury, the effects of methylmercury on dissociated rat cerebellar neurons were examined using a flow cytometer, a confocal laser microscope and three fluorescent dyes, fluo-3 for monitoring the changes in intracellular Ca2+ concentration ([Ca2+](i)) and for detecting live neurons, ethidium for assessing the neurons that are dead or have compromised membranes, and 5-chloromethylfluorescein (CMF) for estimating the cellular content of nonprotein thiols. Methylmercury at concentrations of 1 μM or greater increased the [Ca2+](i) of almost all neurons. Prolonged exposure to methylmercury (3 and 10 μM) produced a further increase in [Ca2+](i), in association with compromising membranes in some neurons. Thereafter, methylmercury induced blebs on membranes of some neurons with increased [Ca2+](i). Methylmercury at concentrations of 0.3 μM or greater dose-dependently decreased the cellular content of nonprotein thiols. Results suggest that methylmercury may induce the loss of membrane integrity through destabilized Ca2+ homeostasis and oxidative stress in mammalian brain neurons.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalEnvironmental Toxicology and Pharmacology
Volume3
Issue number4
DOIs
Publication statusPublished - 1997 Sep 1

Keywords

  • Cell membrane
  • Cerebellar neurons
  • Flow cytometer
  • Fluorescent dyes
  • Intercellular Ca
  • Methylmercury

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Fluorescent estimation on cytotoxicity of methylmercury in dissociated rat cerebellar neurons: Its comparison with ionomycin'. Together they form a unique fingerprint.

Cite this