Fluorescence quenching by complex of a dna aptamer and porphyrin for sensitive detection of porphyrins by capillary electrophoresis

Mari Onodera, Kenji Sueyoshi, Mitsuo Umetsu

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we used a fluorophore-bound, porphyrin-targeted DNA aptamer to increase the sensitivity of capillary electrophoresis for the detection of a model porphyrin. The complex of the DNA aptamer with the porphyrin induced the transfer of energy from the fluorophore to the porphyrin, resulting in the quenching of fluorescence. Monitoring of the quenching of fluorescence by capillary electrophoresis allowed the model porphyrin to be detected at sub-nanomolar concentrations, which is a marked improvement over conventional direct-detection capillary electrophoresis approaches.

Original languageEnglish
Pages (from-to)949-952
Number of pages4
JournalChemistry Letters
Volume50
Issue number5
DOIs
Publication statusPublished - 2021 May

Keywords

  • Capillary electrophoresis
  • DNA aptamer
  • Porphyrin

ASJC Scopus subject areas

  • Chemistry(all)

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