Fluorescence Polarization Measurement System Using a Liquid Crystal Layer and an Image Sensor

Osamu Wakao; Yusaku Fujii; Masatoshi Maeki; Akihiko Ishida; Hirofumi Tani; Akihide Hibara; Manabu Tokeshi

doi:10.1021/acs.analchem.5b01164

Fluorescence Polarization Measurement System Using a Liquid Crystal Layer and an Image Sensor

Osamu Wakao, Yusaku Fujii, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.

Original language	English
Pages (from-to)	9647-9652
Number of pages	6
Journal	Analytical Chemistry
Volume	87
Issue number	19
DOIs	https://doi.org/10.1021/acs.analchem.5b01164
Publication status	Published - 2015 Sep 21
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.",

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AU - Wakao, Osamu

AU - Fujii, Yusaku

AU - Maeki, Masatoshi

AU - Ishida, Akihiko

AU - Tani, Hirofumi

AU - Hibara, Akihide

AU - Tokeshi, Manabu

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