TY - JOUR
T1 - Fluorescence Polarization Measurement System Using a Liquid Crystal Layer and an Image Sensor
AU - Wakao, Osamu
AU - Fujii, Yusaku
AU - Maeki, Masatoshi
AU - Ishida, Akihiko
AU - Tani, Hirofumi
AU - Hibara, Akihide
AU - Tokeshi, Manabu
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/9/21
Y1 - 2015/9/21
N2 - The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.
AB - The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.
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U2 - 10.1021/acs.analchem.5b01164
DO - 10.1021/acs.analchem.5b01164
M3 - Article
C2 - 26352939
AN - SCOPUS:84942855914
VL - 87
SP - 9647
EP - 9652
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 19
ER -