Fluorescence polarization imaging for multisample immunoassay

Osamu Wakao, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper reports a unique fluorescence polarization (FP) imaging system with a microchip applicable to multisample simultaneous FP immunoassay. The measurement principle of the system has already been reported [1]. In this work, we present a FP imaging immunoassay for multisample of physiologically active substances (prostaglandin E2, PGE2). FP signals of all samples in the captured by an image sensor were imaged simultaneously, and their values were acquired separately from the image analysis. The analytical performance of this assay system was comparable to that of conventional apparatus. The result is the first demonstration of the multisample FP imaging immunoassay.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1816-1818
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Fluorescence Polarization
  • Fluorescence Polarization Immunoassay
  • Imaging Immunoassay

ASJC Scopus subject areas

  • Control and Systems Engineering

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