Abstract
We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.
Original language | English |
---|---|
Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 1400-1401 |
Number of pages | 2 |
ISBN (Electronic) | 9780979806490 |
Publication status | Published - 2016 |
Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
---|---|
Country/Territory | Ireland |
City | Dublin |
Period | 16/10/9 → 16/10/13 |
Keywords
- Fluorescence polarization
- Fluorescence polarization immunoassay
- High-throughput immunoassay
ASJC Scopus subject areas
- Control and Systems Engineering