Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis

O. Wakao, M. Maeki, A. Ishida, H. Tani, Akihide Hibara, M. Tokeshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1400-1401
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Fluorescence polarization
  • Fluorescence polarization immunoassay
  • High-throughput immunoassay

ASJC Scopus subject areas

  • Control and Systems Engineering

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