Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis

O. Wakao; M. Maeki; A. Ishida; H. Tani; Akihide Hibara; M. Tokeshi

Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis

O. Wakao, M. Maeki, A. Ishida, H. Tani, Akihide Hibara, M. Tokeshi

Division of Measurements

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1400-1401
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	16/10/9 → 16/10/13

Keywords

Fluorescence polarization
Fluorescence polarization immunoassay
High-throughput immunoassay

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Wakao, O., Maeki, M., Ishida, A., Tani, H., Hibara, A., & Tokeshi, M. (2016). Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1400-1401). Chemical and Biological Microsystems Society.

Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis. / Wakao, O.; Maeki, M.; Ishida, A.; Tani, H.; Hibara, Akihide; Tokeshi, M.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1400-1401.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Wakao, O, Maeki, M, Ishida, A, Tani, H, Hibara, A & Tokeshi, M 2016, Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 1400-1401, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Wakao, O. ; Maeki, M. ; Ishida, A. ; Tani, H. ; Hibara, Akihide ; Tokeshi, M. / Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 1400-1401

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abstract = "We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.",

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AU - Hibara, Akihide

AU - Tokeshi, M.

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N2 - We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.

AB - We report a new high-throughput homogeneous assay format using a microchamber array towards molecular interactions analysis. The assay format is based on the fluorescence polarization (FP) detection using liquid-crystal display (LCD) that can switches a direction of FP by changing its images. In this work, we demonstrate a multiple sample FP immunoassay (FPIA) of prostaglandin E2 in a microchamber array to simultaneously analyze 25 samples. FP signals were imaged simultaneously and their values were acquired separately. This result showed our format with high-throughput had a measurement performance comparable to that of a conventional apparatus designed to single analysis.

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KW - Fluorescence polarization immunoassay

KW - High-throughput immunoassay

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Fluorescence polarization-based multiple samples detection using microchamber array towards high-throughput molecular interaction analysis

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Keywords

ASJC Scopus subject areas

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