Fluorescence lifetimes of benzofurazan adducts with thiol groups in biomolecules

Yuko Yoshikawa, Mamoru Fujitsuka, Akira Watanabe, Osamu Ito, Eisuke Sato, Hiroshi Kokubun

Research output: Contribution to journalArticlepeer-review


Fluorescence lifetimes have been measured for the adducts of ammonium 4-chloro-2,1,3-benzoxadiazole-7-sulfonate, which is known as a fluorescence probe specific to thiol groups. Although the adduct with simple molecule such as mercaptoethanol showed a single exponential fluorescence decay, most adducts with amino acid, peptide, coenzyme and protein showed the bi exponential decay. The fluorescence lifetimes and ratio of the short lifetime fraction to long lifetime fraction of the SBD adducts with biomolecules vary with the molecular size, solvent polarity and micro environment around the fluorescence probe.

Original languageEnglish
Pages (from-to)759-766
Number of pages8
Issue number2
Publication statusPublished - 2003 Mar 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry


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