Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence

Nobuhiro Ohta, Takakazu Nakabayashi, Issei Nagao, Masataka Kinjo, Yumiko Aoki, Minoru Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A dramatic change occurs in the cellular microenvironment during cell stress, but it has been difficult to follow these changes in vivo. Here, fluorescence lifetime imaging (FLIM) microscopy has been used to examine stress-induced changes in the microenvironment in a single cell. It is observed that the fluorescence lifetime of HeLa cells expressing an enhanced green fluorescent protein (EGFP)-tudor fusion protein changes under stress. The change in the fluorescence lifetime appears to be due to an alteration in the local electric field in the protein matrix surrounding the chromophore of EGFP. In fact, the fluorescence lifetime of the GFP chromophore in a polyvinyl alcohol film is found to decrease in the presence of an electric field, based on the measurements of the field-induced change in the fluorescence decay profile. The results indicate that the rate of the non-radiative process of the chromophore of GFP is enhanced by an applied electric field. The FLIM method allows noninvasive determination of the status of the individual cells.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
DOIs
Publication statusPublished - 2009 Jun 15
Externally publishedYes
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications - San Jose, CA, United States
Duration: 2009 Jan 262009 Jan 29

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7190
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
CountryUnited States
CitySan Jose, CA
Period09/1/2609/1/29

Keywords

  • Cell stress
  • EGFP-tudor fusion protein
  • Electric field effect
  • Enhanced green fluorescent protein (EGFP)
  • Fluorescence Lifetime Imaging (FLIM)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ohta, N., Nakabayashi, T., Nagao, I., Kinjo, M., Aoki, Y., & Tanaka, M. (2009). Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications [71900R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7190). https://doi.org/10.1117/12.808484