A new type of laser-excited fluorescence microscope system was developed to achieve excellent sensitivity and spatial resolution for a single biological cell utilizing an ultra-high sensitive image camera and digital image processing technique for quantitative three-dimensional intensity display. Using this system, fluorescence intensity distribution across a TE-2 cultured esophagus cancer cell which had uptaken Hematoporphyrin dihydrochloride (Hp. 2HCl) and Hematoporphyrin derivative (HpD) were measured. It was found quantitatively that the fluorescence intensity from both dyes is weak at the central portion and strong around it. This result means that Hp. 2HCl and HpD are mainly uptaken in cytoplasm of this cancer cell, and the photochemical or photodynamic effect which is responsible for photoradiation therapy (PRT) of malignant tumors will take place primarily in this cell sector for the case of Hp uptake.
|Number of pages||2|
|Journal||japanese journal of medical electronics and biological engineering|
|Publication status||Published - 1984 Apr 1|
ASJC Scopus subject areas
- Biomedical Engineering