Fluorescence emission detection of single-nucleotide polymorphisms by a naphthyridine-benzofurazan conjugate.

Hiroyuki Satake, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticle

Abstract

We have successfully developed a class of ligand that exhibits a fluorescence-enhancement upon binding to pyrimidine bases opposite an AP site in DNA duplexes. The present ligand, Naph-c3-DBD, in which DBD (7-N,N-dimethylaminosulfonylbenzo-2-oxa-1,3-diazole) is connected to 2-amino-7-methyl-1,8-naphthyridine by a propylene linker, is capable of selectively binding to pyrimidine bases over purine bases, and the binding event is accompanied by a significant enhancement of emission due to the DBD moiety (emission maximum at 597 nm). The response of the ligand is almost specific to pyrimidine bases, making it possible to detect pyrimidine/purine transversion.

Original languageEnglish
Pages (from-to)297-298
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Medicine(all)

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