TY - JOUR
T1 - Fluorescence emission detection of single-nucleotide polymorphisms by a naphthyridine-benzofurazan conjugate.
AU - Satake, Hiroyuki
AU - Nishizawa, Seiichi
AU - Teramae, Norio
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2007
Y1 - 2007
N2 - We have successfully developed a class of ligand that exhibits a fluorescence-enhancement upon binding to pyrimidine bases opposite an AP site in DNA duplexes. The present ligand, Naph-c3-DBD, in which DBD (7-N,N-dimethylaminosulfonylbenzo-2-oxa-1,3-diazole) is connected to 2-amino-7-methyl-1,8-naphthyridine by a propylene linker, is capable of selectively binding to pyrimidine bases over purine bases, and the binding event is accompanied by a significant enhancement of emission due to the DBD moiety (emission maximum at 597 nm). The response of the ligand is almost specific to pyrimidine bases, making it possible to detect pyrimidine/purine transversion.
AB - We have successfully developed a class of ligand that exhibits a fluorescence-enhancement upon binding to pyrimidine bases opposite an AP site in DNA duplexes. The present ligand, Naph-c3-DBD, in which DBD (7-N,N-dimethylaminosulfonylbenzo-2-oxa-1,3-diazole) is connected to 2-amino-7-methyl-1,8-naphthyridine by a propylene linker, is capable of selectively binding to pyrimidine bases over purine bases, and the binding event is accompanied by a significant enhancement of emission due to the DBD moiety (emission maximum at 597 nm). The response of the ligand is almost specific to pyrimidine bases, making it possible to detect pyrimidine/purine transversion.
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U2 - 10.1093/nass/nrm149
DO - 10.1093/nass/nrm149
M3 - Article
C2 - 18029704
AN - SCOPUS:42949176266
SP - 297
EP - 298
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
SN - 1746-8272
IS - 51
ER -