TY - JOUR
T1 - Fluorescence determination of closed-shell rare earth metal ions by reversed-phase HPLC with precolumn derivatization using 8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetate
AU - Saito, Shingo
AU - Xu, Wen Zhe
AU - Hoshino, Hitoshi
AU - Yotsuyanagi, Takao
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - A sensitive and selective determination of closed-shell rare earth metal ions (La3+, Lu3+, and Y3+) has been demonstrated using a pre-column chelating reagent {8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetatic acid (Quin2)}, in reversed-phase HPLC. Although the resolution is still unsatisfactory in this HPLC system, rare- earth metal chelates with this octadentate aromatic polyaminocarboxylate can be detected. This result suggests that these chelates are unexpectedly inert for the dissociation process during elution. The order of elution is quite different from that of the conventional ion-exchange mode separation. Middle-lanthanide ion-Quin2 chelates were most rapidly eluted, followed by the heavy ones; the light ones were the most strongly retained. Since ligand-centered fluorescence was observed for only La3+, Lu3+, Y3+, and Gd3+-Quin2 chelates, and other rare earth metal ion chelates were sufficiently quenched through a paramagnetic deactivation process, these four metal ions were selectively detected by fluorimetric detection (λex= 335 nm, λem= 500 nm). The detection limits of La3+, Lu3+, and Y3+ were 2.2, 4.0, and 1.9 ppb, respectively, on a 3σ basis. No interference from each 10-fold excess of other rare-earth metal ions was observed. The success of this HPLC system for rare- earth ions is derived from the combined properties of the Quin2 chelates in terms of inertness, Chromatographic retention and fluorescence detection.
AB - A sensitive and selective determination of closed-shell rare earth metal ions (La3+, Lu3+, and Y3+) has been demonstrated using a pre-column chelating reagent {8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetatic acid (Quin2)}, in reversed-phase HPLC. Although the resolution is still unsatisfactory in this HPLC system, rare- earth metal chelates with this octadentate aromatic polyaminocarboxylate can be detected. This result suggests that these chelates are unexpectedly inert for the dissociation process during elution. The order of elution is quite different from that of the conventional ion-exchange mode separation. Middle-lanthanide ion-Quin2 chelates were most rapidly eluted, followed by the heavy ones; the light ones were the most strongly retained. Since ligand-centered fluorescence was observed for only La3+, Lu3+, Y3+, and Gd3+-Quin2 chelates, and other rare earth metal ion chelates were sufficiently quenched through a paramagnetic deactivation process, these four metal ions were selectively detected by fluorimetric detection (λex= 335 nm, λem= 500 nm). The detection limits of La3+, Lu3+, and Y3+ were 2.2, 4.0, and 1.9 ppb, respectively, on a 3σ basis. No interference from each 10-fold excess of other rare-earth metal ions was observed. The success of this HPLC system for rare- earth ions is derived from the combined properties of the Quin2 chelates in terms of inertness, Chromatographic retention and fluorescence detection.
KW - Fluorescence detection
KW - Metal chelate
KW - Quin2
KW - Rare earth metal
KW - Reversed-phase HPLC
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U2 - 10.2116/bunsekikagaku.50.113
DO - 10.2116/bunsekikagaku.50.113
M3 - Article
AN - SCOPUS:0035586503
VL - 50
SP - 113
EP - 117
JO - Bunseki Kagaku
JF - Bunseki Kagaku
SN - 0525-1931
IS - 2
ER -