Fluorescence determination of closed-shell rare earth metal ions by reversed-phase HPLC with precolumn derivatization using 8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetate

Shingo Saito, Wen Zhe Xu, Hitoshi Hoshino, Takao Yotsuyanagi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A sensitive and selective determination of closed-shell rare earth metal ions (La3+, Lu3+, and Y3+) has been demonstrated using a pre-column chelating reagent {8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetatic acid (Quin2)}, in reversed-phase HPLC. Although the resolution is still unsatisfactory in this HPLC system, rare- earth metal chelates with this octadentate aromatic polyaminocarboxylate can be detected. This result suggests that these chelates are unexpectedly inert for the dissociation process during elution. The order of elution is quite different from that of the conventional ion-exchange mode separation. Middle-lanthanide ion-Quin2 chelates were most rapidly eluted, followed by the heavy ones; the light ones were the most strongly retained. Since ligand-centered fluorescence was observed for only La3+, Lu3+, Y3+, and Gd3+-Quin2 chelates, and other rare earth metal ion chelates were sufficiently quenched through a paramagnetic deactivation process, these four metal ions were selectively detected by fluorimetric detection (λex= 335 nm, λem= 500 nm). The detection limits of La3+, Lu3+, and Y3+ were 2.2, 4.0, and 1.9 ppb, respectively, on a 3σ basis. No interference from each 10-fold excess of other rare-earth metal ions was observed. The success of this HPLC system for rare- earth ions is derived from the combined properties of the Quin2 chelates in terms of inertness, Chromatographic retention and fluorescence detection.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalBUNSEKI KAGAKU
Volume50
Issue number2
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Fluorescence detection
  • Metal chelate
  • Quin2
  • Rare earth metal
  • Reversed-phase HPLC

ASJC Scopus subject areas

  • Analytical Chemistry

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