Fluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model

Naoya Sakamoto, Yosuke Ueki, Masaki Oi, Takuya Kiuchi, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The adhesion and migration of leukocytes to arterial endothelial cells (ECs), one of the indicators of early atherogenesis, is believed to be correlated with the blood flow conditions and interactions between vascular cells including vascular smooth muscle cells (SMCs). In this study, we investigated the effect of fluid shear stress on the transendothelial migration of leukocytes in a coculture model (CM) composed of human umbilical ECs and SMCs, a layer of collagen type I, and a porous membrane. Following exposure to a fluid shear stress of 1.5 Pa for 24 h, human mononuclear leukocytes were seeded on the EC surface and cultured for 1 h. Leukocytes migrating across the EC layer were observed by confocal laser scanning microscopy. The number of migrating leukocytes in the statically cultured CM was significantly larger than that in the static EC monoculture model. The exposure to the shear stress significantly decreased the leukocyte migration induced by the coculture condition. In the static CM, fluorescence staining and Western blotting showed a higher expression of intercellular adhesion molecule-1 (ICAM-1) of ECs. These results indicate that SMC-derived bioactive soluble factors may stimulate the ICAM-1 expression of cocultured ECs, possibly leading to leukocyte migration into the subendothelial space.

Original languageEnglish
Pages (from-to)403-408
Number of pages6
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 2018 Jul 20
Externally publishedYes


  • Adhesion molecules
  • Atherosclerosis
  • Coculture system
  • Endothelial–smooth muscle cell interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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