Fluid dynamics and electrical detection of λDNA in electrode-embedded nanochannels

Satoshi Uehara, Makusu Tsutsui, Kentaro Doi, Masateru Taniguchi, Satoyuki Kawano, Tomoji Kawai

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In the present study, we address theoretical approaches for the experimental results to investigate the flow dynamics of λDNA through a nanochannel in which two nanoelectrodes are integrated. In order to elucidate the relationship between the longitudinal ionic current and the electrophoresis of λDNA in the specific micro/nanofluidics, we develop a theoretical model for the macroscopic fluid dynamics in a Lagrangian framework. The measured current change associated with a single molecule translocation through the channel is explained by the principle of the Coulter counter that allowed to predict the conformation of λDNA. We also analyze the local velocity of λDNA passing through a nanoscaled confined channel. A result from the model is in considerable agreement with the experimental observations for the electrophoretic flow of λDNA. The basic knowledge obtained here may be useful in developing electrical methods for controlling the electrophoretic velocity of single-molecule DNA for realizing the nanopore sequencer.

Original languageEnglish
Pages (from-to)244-256
Number of pages13
JournalJournal of Biomechanical Science and Engineering
Volume7
Issue numberSPECIALISSUE.3
Publication statusPublished - 2012 Dec 1
Externally publishedYes

Keywords

  • Biomacromorecule
  • DNA sequencer
  • Electrophoresis
  • Nanofluidics
  • Single molecule observation

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Fluid dynamics and electrical detection of λDNA in electrode-embedded nanochannels'. Together they form a unique fingerprint.

Cite this