Fluid dynamics alter caenorhabditis elegans body length via TGF-β/DBL-1 neuromuscular signaling

Shunsuke Harada, Toko Hashizume, Kanako Nemoto, Zhenhua Shao, Nahoko Higashitani, Timothy Etheridge, Nathaniel J. Szewczyk, Keiji Fukui, Akira Higashibata, Atsushi Higashitani

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Skeletal muscle wasting is a major obstacle for long-term space exploration. Similar to astronauts, the nematode Caenorhabditis elegans displays negative muscular and physical effects when in microgravity in space. It remains unclear what signaling molecules and behavior(s) cause these negative alterations. Here we studied key signaling molecules involved in alterations of C. elegans physique in response to fluid dynamics in ground-based experiments. Placing worms in space on a 1G accelerator increased a myosin heavy chain, myo-3, and a transforming growth factor-β (TGF-β), dbl-1, gene expression. These changes also occurred when the fluid dynamic parameters viscosity/drag resistance or depth of liquid culture were increased on the ground. In addition, body length increased in wild type and body wall cuticle collagen mutants, rol-6 and dpy-5, grown in liquid culture. In contrast, body length did not increase in TGF-β, dbl-1, or downstream signaling pathway, sma-4/Smad, mutants. Similarly, a D1-like dopamine receptor, DOP-4, and a mechanosensory channel, UNC-8, were required for increased dbl-1 expression and altered physique in liquid culture. As C. elegans contraction rates are much higher when swimming in liquid than when crawling on an agar surface, we also examined the relationship between body length enhancement and rate of contraction. Mutants with significantly reduced contraction rates were typically smaller. However, in dop-4, dbl-1, and sma-4 mutants, contraction rates still increased in liquid. These results suggest that neuromuscular signaling via TGF-β/DBL-1 acts to alter body physique in response to environmental conditions including fluid dynamics.

    Original languageEnglish
    Article number16006
    Journalnpj Microgravity
    Volume2
    DOIs
    Publication statusPublished - 2016 Jan 7

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Materials Science (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology (miscellaneous)
    • Agricultural and Biological Sciences (miscellaneous)
    • Physics and Astronomy (miscellaneous)
    • Space and Planetary Science

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