Flower form alteration by genetic transformation with the class B MADS-box genes of Agapanthus praecox spp. orientalis in transgenic dicot and monocot plants

Masaru Nakano, Hiroto Umehara, Yoshihiro Hara, Motohide Makino, Mika Igarashi, Mutsumi Nakada, Toru Nakamura, Yoichiro Hoshino, Akira Kanno

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The class B genes, which belong to the MADS-box gene family, play important roles in regulating petal and stamen development in flowering plants. These genes exist in two different types termed DEF- and GLO-like genes, and the B-function is provided by heterodimers of a DEF- and a GLO-like gene product. In the present study, dicot (tobacco and lettuce) and monocot (Tricyrtis hirta) plants were transformed with the GLO-like gene of Agapanthus praecox ssp. orientalis ApGLO alone or in combination with the DEF-like gene of the same plant ApDEF. In two out of 10 transgenic tobacco plants containing ApGLO, sepals partially converted into petaloid organs. For lettuce, ray florets of four out of nine transgenic plants containing ApGLO also developed additional petaloid organs. In two out of five transgenic T. hirta plants containing both ApGLO and ApDEF, organs developed in whorl 4 showed noticeable morphological alteration: they were much longer compared with carpels of non-transgenic plants, and had purple spots overall on the surface as filaments of non-transgenic plants. No morphological alterations were observed in vegetative organs between transgenic and non-transgenic plants for all the three species. The results obtained in the present study indicate a possibility of molecular breeding for flower form alteration by genetic transformation with the class B MADS-box gene(s) of heterologous plant species.

Original languageEnglish
Pages (from-to)425-429
Number of pages5
JournalMolecular Breeding
Volume20
Issue number4
DOIs
Publication statusPublished - 2007 Nov 1

Keywords

  • Agapanthus
  • Flower form alteration
  • Lettuce
  • MADS-box gene
  • Tobacco
  • Transgenic plant
  • Tricyrtis

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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