Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle

Shinji Deguchi, Kenjiro Maeda, Toshiro Ohashi, Masaaki Sato

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The mechanical contribution of nucleus in adherent cells to bearing intracellular stresses remains unclear. In this paper, the effects of fluid shear stress on morphology and elastic properties of endothelial nuclei were investigated. The morphological observation suggested that the nuclei in the cytoplasm were being vertically compressed under static conditions, whereas they were elongated and more compressed with a fluid shear stress of 2 Pa (20 dyn/cm2) onto the cell. The elongated nuclei remained the shape even after they were isolated from the cells. The micropipette aspiration technique on the isolated nuclei revealed that the elastic modulus of elongated nuclei, 0.62±0.15 kPa (n=13, mean±SD), was significantly higher than that of control nuclei, 0.42±0.12 kPa (n=11), suggesting that the nuclei remodeled their structure due to the shear stress. Based of these results and a transmission electron microscopy, a possibility of the nucleus as an intracellular compression-bearing organelle was proposed, which will impact interpretation of stress distribution in adherent cells.

Original languageEnglish
Pages (from-to)1751-1759
Number of pages9
JournalJournal of Biomechanics
Volume38
Issue number9
DOIs
Publication statusPublished - 2005 Sep

Keywords

  • Cell mechanics
  • Mechanical properties
  • Mechanotransduction
  • Nucleus
  • Shear stress

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

Fingerprint Dive into the research topics of 'Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle'. Together they form a unique fingerprint.

  • Cite this