Flow Cytometric DNA Analysis of Lung Cancer Cell Lines

Shinchiro Okada, Shunsuke Kobayashi, Hirohisa Inaba, Nobuyuki Sato, Toru Hasumi, Wataru Shoji, Hiroyuki Yoshida, Shigefumi Fujimura

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor DNA content (ploidy) was analyzed by use of flow cytometry (FCM) in 17 lung cancer cell lines which were subcultured in our laboratory. The study included 6 adenocarcinomas, 2 squamous cell carcinomas, 1 adenosquamous cell carcinoma, 5 large cell carcinomas, and 3 small cell carcinomas. Of the 17 lung carcinoma cell lines, 15 revealed aneuploid patterns with DNA index above 1.1, whereas one had diploid. The mean DNA index (DI) in adenocarcinoma, was 1.34±0.09, DI 1.6, in squamous cell carcinoma, DI 1.0 in adenosquamous cell carcinoma, DI 1.70±0.66 in large cell carcinoma, and DI 1.29 in small cell carcinoma. Of the 17 cell lines, three lines showed multiploid patterns with clinically poor prognosis and indicated heterogeneity. Flow cytometric DNA analysis using lung cancer cell lines could provide further basic study of lung cancer cells and give a useful information on the degree of the malignancy clinically.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalTohoku Journal of Experimental Medicine
Volume168
Issue number2
DOIs
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • DNA index
  • flow cytometry
  • in vitro
  • lung cancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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