Flow cytometric analysis on cytotoxic action of amyloid β protein fragment 25-35 on brain neurons dissociated from the rats

Katsutoshi Furukawa, Yasuo Oyama, Lumi Chikahisa, Yoshiko Hatakeyama, Norio Akaike

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Effects of amyloid β protein fragment 25-35, AβP(25-35), on membrane permeability and cell viability were examined in the brain neurons dissociated from the rats using a flow cytometer and two fluorescent dyes, fluo-3 to monitor intracellular Ca2+ concentration ([Ca2+]i) of neurons and ethidium which is impermeant to membranes of intact neurons to stain dead and dying neurons. AβP(25-35) augmented fluo-3 fluorescence of some neurons at concentrations greater than 1 μM, indicating an increase in [Ca2+]i although other neurons (about 80% of total neurons) did not respond to AβP(25-35) event at 10 μM. AβP(25-35) at 1 μM or greater increased dose-dependently the number of ethidium-stained neurons, suggesting a dose-dependent increase in number of dead and dying neurons. Results suggest that AβP(25-35) increases the membrane permeability of brain neurons, resulting in a destabilized intracellular homeostasis that leads to neuonal death.

Original languageEnglish
Pages (from-to)259-262
Number of pages4
JournalBrain research
Volume662
Issue number1-2
DOIs
Publication statusPublished - 1994 Oct 31

Keywords

  • Amyloid protein fragment
  • Brain neuron
  • Cytotoxicity
  • Ethidium bromide
  • Flow cytometer

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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