Flavodiiron protein substitutes for cyclic electron flow without competing CO2 assimilation in rice

Shinya Wada, Hiroshi Yamamoto, Yuji Suzuki, Wataru Yamori, Toshiharu Shikanai, Amane Makino

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Flavodiiron protein (FLV) mediates photoreduction of O2to H2O. It is conserved from cyanobacteria to gymnosperms but not in angiosperms. The introduction of a moss (Physcomitrella patens) FLV (PpFLV) gene into Arabidopsis (Arabidopsis thaliana) made photosystem I (PSI) resistant to fluctuating light. Here, we used the same strategy with three rice (Oryza sativa) genotypes. PpFLV in the wild-type rice background functioned as an efficient PSI electron sink and increased resistance to PSI photodamage under fluctuating light. The introduction of PpFLV into the PGR5-RNAi mutant [defective in PROTON GRADIENT REGULATION5 (PGR5)-dependent cyclic electron transport around PSI, CET-PSI], the crr6 mutant [defective in chloroplast NAD(P)H-dehydrogenase-like complex (NDH)-dependent CET-PSI], and the PGR5-RNAi crr6 double mutant (double defective in CET-PSI activity) alleviated PSI photodamage under fluctuating light. Furthermore, PpFLV substituted for the function of PGR5- and NDH-dependent CET-PSI without competing for CO2 assimilation under constant light, as there was no difference in CO2 assimilation per Rubisco content and biomass production was recovered to the wild-type level. Thus, the exogenous FLV system could act not only as a safety valve under fluctuating light, but also generate a proton motive force for balancing the ATP/NADPH production ratio during steady-state photosynthesis.

Original languageEnglish
Pages (from-to)1509-1518
Number of pages10
JournalPlant physiology
Volume176
Issue number2
DOIs
Publication statusPublished - 2018 Feb

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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