Flagellin/TLR5 signaling potentiates airway serous secretion from swine tracheal submucosal glands

Soshi Muramatsu, Tsutomu Tamada, Masayuki Nara, Koji Murakami, Toshiaki Kikuchi, Masahiko Kanehira, Yoshio Maruyama, Masahito Ebina, Toshihiro Nukiwa, Masakazu Ichinose

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Airway serous secretion is essential for the maintenance of mucociliary transport in airway mucosa, which is responsible for the upregulation of mucosal immunity. Although there are many articles concerning the importance of Tolllike receptors (TLRs) in airway immune systems, the direct relationship between TLRs and airway serous secretion has not been well investigated. Here, we focused on whether TLR5 ligand flagellin, which is one of the components of Pseudomonas aeruginosa, is involved in the upregulation of airway serous secretion. Freshly isolated swine tracheal submucosal gland cells were prepared, and the standard patch-clamp technique was applied for measurements of the whole cell ionic responses of these cells. Flagellin showed potentiating effects on these oscillatory currents induced by physiologically relevant low doses of acetylcholine (ACh) in a dose-dependent manner. These potentiating effects were TLR5 dependent but TLR4 independent. Both nitric oxide (NO) synthase inhibitors and cGMPdependent protein kinase (cGK) inhibitors abolished these flagellininduced potentiating effects. Furthermore, TLR5 was abundantly expressed on tracheal submucosal glands. Flagellin/TLR5 signaling further accelerated the intracellular NO synthesis induced by ACh. These findings suggest that TLR5 takes part in the airway mucosal defense systems as a unique endogenous potentiator of airway serous secretions and that NO/cGMP/cGK signaling is involved in this rapid potentiation by TLR5 signaling.

Original languageEnglish
Pages (from-to)L819-L830
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume305
Issue number11
DOIs
Publication statusPublished - 2013 Dec 1

Keywords

  • Ca-activated Cl channel
  • Nitric oxide
  • Patch-clamp
  • Pseudomonas aeruginosa
  • cGMP-dependent protein kinase

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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