Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly

Alexandra Faulds-Pain, Christopher Birchall, Christine Aldridge, Wendy D. Smith, Giulia Grimaldi, Shuichi Nakamura, Tomoko Miyata, Joe Gray, Guanglai Li, Jay X. Tang, Keiichi Namba, Tohru Minamino, Phillip D. Aldridge

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in ΔfljK ΔfljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament.

Original languageEnglish
Pages (from-to)2695-2707
Number of pages13
JournalJournal of bacteriology
Volume193
Issue number11
DOIs
Publication statusPublished - 2011 Jun

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Faulds-Pain, A., Birchall, C., Aldridge, C., Smith, W. D., Grimaldi, G., Nakamura, S., Miyata, T., Gray, J., Li, G., Tang, J. X., Namba, K., Minamino, T., & Aldridge, P. D. (2011). Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly. Journal of bacteriology, 193(11), 2695-2707. https://doi.org/10.1128/JB.01172-10