TY - JOUR
T1 - Flagellin redundancy in Caulobacter crescentus and its implications for flagellar filament assembly
AU - Faulds-Pain, Alexandra
AU - Birchall, Christopher
AU - Aldridge, Christine
AU - Smith, Wendy D.
AU - Grimaldi, Giulia
AU - Nakamura, Shuichi
AU - Miyata, Tomoko
AU - Gray, Joe
AU - Li, Guanglai
AU - Tang, Jay X.
AU - Namba, Keiichi
AU - Minamino, Tohru
AU - Aldridge, Phillip D.
PY - 2011/6
Y1 - 2011/6
N2 - Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in ΔfljK ΔfljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament.
AB - Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in ΔfljK ΔfljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament.
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U2 - 10.1128/JB.01172-10
DO - 10.1128/JB.01172-10
M3 - Article
C2 - 21441504
AN - SCOPUS:79958158232
VL - 193
SP - 2695
EP - 2707
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 11
ER -