FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Yoshiyuki Manabe; Makoto Mukai; Satoko Ito; Nobuki Kato; Minoru Ueda

doi:10.1039/b915843j

FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Yoshiyuki Manabe, Makoto Mukai, Satoko Ito, Nobuki Kato, Minoru Ueda

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 × 10⁵ differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.

Original language	English
Pages (from-to)	469-471
Number of pages	3
Journal	Chemical Communications
Volume	46
Issue number	3
DOIs	https://doi.org/10.1039/b915843j
Publication status	Published - 2010

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b915843j

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FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae. / Manabe, Yoshiyuki; Mukai, Makoto; Ito, Satoko; Kato, Nobuki ; Ueda, Minoru.

In: Chemical Communications, Vol. 46, No. 3, 2010, p. 469-471.

Research output: Contribution to journal › Article › peer-review

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AU - Manabe, Yoshiyuki

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AU - Ito, Satoko

AU - Kato, Nobuki

AU - Ueda, Minoru

PY - 2010

Y1 - 2010

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AB - We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 × 105 differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.

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FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

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