Fission yeast Swi5 protein, a novel DNA recombination mediator

Nami Haruta; Yufuko Akamatsu; Yasuhiro Tsutsui; Yumiko Kurokawa; Yasuto Murayama; Benoit Arcangioli; Hiroshi Iwasaki

doi:10.1016/j.dnarep.2007.07.004

Fission yeast Swi5 protein, a novel DNA recombination mediator

Nami Haruta, Yufuko Akamatsu, Yasuhiro Tsutsui, Yumiko Kurokawa, Yasuto Murayama, Benoit Arcangioli, Hiroshi Iwasaki

Research output: Contribution to journal › Review article › peer-review

18 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	DNA Repair
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.dnarep.2007.07.004
Publication status	Published - 2008 Jan 1
Externally published	Yes

Keywords

Dmc1
Homologous recombination
Rad51
Recombination mediator
Schizosaccharomyces pombe
Swi5-Sfr1

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Fission yeast Swi5 protein, a novel DNA recombination mediator",

abstract = "The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.",

keywords = "Dmc1, Homologous recombination, Rad51, Recombination mediator, Schizosaccharomyces pombe, Swi5-Sfr1",

author = "Nami Haruta and Yufuko Akamatsu and Yasuhiro Tsutsui and Yumiko Kurokawa and Yasuto Murayama and Benoit Arcangioli and Hiroshi Iwasaki",

year = "2008",

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doi = "10.1016/j.dnarep.2007.07.004",

language = "English",

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journal = "DNA Repair",

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T1 - Fission yeast Swi5 protein, a novel DNA recombination mediator

AU - Haruta, Nami

AU - Akamatsu, Yufuko

AU - Tsutsui, Yasuhiro

AU - Kurokawa, Yumiko

AU - Murayama, Yasuto

AU - Arcangioli, Benoit

AU - Iwasaki, Hiroshi

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.

AB - The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.

KW - Dmc1

KW - Homologous recombination

KW - Rad51

KW - Recombination mediator

KW - Schizosaccharomyces pombe

KW - Swi5-Sfr1

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DO - 10.1016/j.dnarep.2007.07.004

M3 - Review article

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AN - SCOPUS:36549080101

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JO - DNA Repair

JF - DNA Repair

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