TY - JOUR
T1 - Fission yeast Swi5 protein, a novel DNA recombination mediator
AU - Haruta, Nami
AU - Akamatsu, Yufuko
AU - Tsutsui, Yasuhiro
AU - Kurokawa, Yumiko
AU - Murayama, Yasuto
AU - Arcangioli, Benoit
AU - Iwasaki, Hiroshi
N1 - Funding Information:
Our study was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology (MECSST) of Japan and from the Japan Society for the Promotion of Science (JSPS), and by a grant for the 2005 Strategic Research Project (no. K17005) of Yokohama City University. NH was supported by JSPS fellowships for young scientists.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.
AB - The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.
KW - Dmc1
KW - Homologous recombination
KW - Rad51
KW - Recombination mediator
KW - Schizosaccharomyces pombe
KW - Swi5-Sfr1
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U2 - 10.1016/j.dnarep.2007.07.004
DO - 10.1016/j.dnarep.2007.07.004
M3 - Review article
C2 - 17716957
AN - SCOPUS:36549080101
VL - 7
SP - 1
EP - 9
JO - DNA Repair
JF - DNA Repair
SN - 1568-7864
IS - 1
ER -