Fission yeast Swi5 protein, a novel DNA recombination mediator

Nami Haruta, Yufuko Akamatsu, Yasuhiro Tsutsui, Yumiko Kurokawa, Yasuto Murayama, Benoit Arcangioli, Hiroshi Iwasaki

Research output: Contribution to journalReview articlepeer-review

19 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalDNA Repair
Volume7
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1
Externally publishedYes

Keywords

  • Dmc1
  • Homologous recombination
  • Rad51
  • Recombination mediator
  • Schizosaccharomyces pombe
  • Swi5-Sfr1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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