Fine-scale dispersal in a stream caddisfly inferred from spatial autocorrelation of microsatellite markers

Sakiko Yaegashi, Kozo Watanabe, Michael T. Monaghan, Tatsuo Omura

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Most population genetic studies in streams infer long-term patterns of gene flow by calculating fixation indices (e.g., F ST) among sampled populations. In more-recent analytical methods, the need to assign individuals to populations a priori (clustering algorithms) is relaxed, and spatial autocorrelation analysis of allele frequencies (SA) is used to infer finer-scale and potentially short-term dispersal distances. We applied multiple methods to study the population genetic structure of the riverine caddisfly Stenopsyche marmorata (Trichoptera:Stenopsychidae) from 4 adjacent catchments in northeastern Japan. We genotyped larval individuals (N = 532) from 30 sites at 8 polymorphic microsatellite loci. Fixation indices suggested low levels of genetic differentiation among populations (global F ST = 0.062, p < 0.01), and significant isolation-by-distance (IBD) indicated populations were in drift-migration equilibrium. Bayesian clustering separated S. marmorata into distinct upland (>250 m asl) and lowland populations, with different F ST values (upland F ST = 0.048, p < 0.01; lowland F ST = 0.029, p < 0.01) and significant IBD only among upland populations. Allele frequencies were significantly positively autocorrelated (Moran's I > 0, p < 0.05) at distances up to 18 km along streams and up to 12 km across terrestrial habitat. These values were similar to directly observed flight distance in a single generation for this species in the field. We conclude that the multiple-method approach revealed: 1) unexpected population subdivision between upland and lowland areas that may result from local adaptation, differences in phenology, and historical colonization by multiple lineages; and 2) fine-scale estimates of dispersal that match direct observations of flight and suggest gene flow is more pronounced along water courses in this species.

Original languageEnglish
Pages (from-to)172-180
Number of pages9
JournalFreshwater Science
Volume33
Issue number1
DOIs
Publication statusPublished - 2014 Mar

Keywords

  • Isolation by Distance
  • aquatic
  • catchment
  • freshwater
  • insect
  • river
  • stream

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Aquatic Science

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