Fine mapping of a major quantitative trait locus, qLG-9, that controls seed longevity in rice (Oryza sativa L.)

K. Sasaki, Y. Takeuchi, K. Miura, T. Yamaguchi, T. Ando, T. Ebitani, A. Higashitani, T. Yamaya, M. Yano, T. Sato

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Abstract: A quantitative trait locus, qLG-9, for seed longevity in rice has previously been detected on chromosome 9 by using backcross inbred lines derived from a cross between Japonica-type (Nipponbare) and Indica-type (Kasalath) cultivars. In the present study, the chromosomal location of qLG-9 was precisely determined by fine-scale mapping. Firstly, allelic difference in qLG-9 was verified by QTL analysis of an F2 population derived from a cross between Nipponbare and NKSL-1, in which a segment of Kasalath chromosome 9 was substituted in Nipponbare genetic background. Then, we selected F2 plants in which recombination had occurred near qLG-9 and performed F3 progeny testing on these plants to determine the genotype classes of qLG-9. Eventually, qLG-9 was mapped in a 30-kb interval (defined by two markers, CAPSb and CHPa12) of the Nipponbare genome sequence. This allowed us to nominate positional candidate genes of qLG-9. Additionally, we developed near-isogenic lines (NIL) for qLG-9 by marker-assisted selection. qLG-9 NIL showed significantly higher seed longevity than isogenic control of Nipponbare. These results will facilitate cloning of the gene(s) underlying qLG-9 as well as marker-assisted transfer of desirable genes for seed longevity improvement in rice.

Key message: We fine-mapped a quantitative trait locus,qLG-9, for seed longevity detected between Japonica-type and Indica-type cultivars.qLG-9was mapped in a 30-kb interval of the Nipponbare genome sequence.

Original languageEnglish
Pages (from-to)769-778
Number of pages10
JournalTheoretical and Applied Genetics
Volume128
Issue number4
DOIs
Publication statusPublished - 2015 Apr

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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