Fimbria‐specific immune response in various inbred mice inoculated with Porphyromonas gingivalis 381

E. Isogai, H. Isogai, S. Takagi, N. Ishii, N. Fujii, K. Kimura, M. Hayashi, F. Yoshimura

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    12 Citations (Scopus)

    Abstract

    We studied the genetic control of Porphyromonas gingivalis fimbriae response. Inbred mice with different H‐2 haplotypes and/or different genetic backgrounds were inoculated with viable P. gingivalis 381 cells and tested for fimbria‐specific T cell responses in vivo (delayed‐type hypersensitivity). H‐2d mice showed a strong footpad response, whereas H‐2b mice showed a weak response to fimbriae from P. gingivalis. Similar evidence of genetic control was obtained with an enzyme‐linked immunosorbent assay for the detection of IgG antibody in inbred mice (BALB/c, C3H/HeN and C57BL/6). Several immunoglobulin G (IgG) subclass responses were associated with H‐2 in these B10 congenic mice. However, quantification of IgG antibody to fimbriae was not controlled by H‐2 in B10 congenic mice. The results indicate that, in mice, the responsiveness to fimbriae of P. gingivalis can be controlled by several genes, including the H‐2 complex. C3H/HeN mice were inoculated with the fimbriae intravenously, and the expression of surface antigens on spleen T cells was measured in a fluorescent antibody cell sorter. Stimulation by fimbriae resulted in a changed expression of surface antigens on T cells. Thus, the fimbriae can induce T cell activation.

    Original languageEnglish
    Pages (from-to)118-122
    Number of pages5
    JournalOral Microbiology and Immunology
    Volume9
    Issue number2
    DOIs
    Publication statusPublished - 1994 Apr

    Keywords

    • H‐2 haplotype
    • Porphyromonas gingivalis
    • fimbriae
    • immune response

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Dentistry(all)
    • Microbiology (medical)

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