Fibroblast-migration in a wound model of ascorbic acid-supplemented three-dimensional culture system: The effects of cytokines and malotilate, a new wound healing stimulant, on cell-migration

Osamu Ohgoda, Atsushi Sakai, Hiroyasu Koga, Kazuo Kanai, Tsuneo Miyazaki, Yoshimi Niwano

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23 Citations (Scopus)

Abstract

To assess the migratory response of fibroblasts in vitro, normal human dermal fibroblasts (NHDF) were cultured in the presence of L-ascorbic acid 2- phosphate to induce a multilayered structure. Round wounds were made by punching, and the migratory response was evaluated by counting the number of migrating cells in the wounded areas. Collagenase activity in the culture- medium was then measured. When the wound model was treated with bFGF, IL-1α or PDGF, the migratory response was facilitated with increased collagenase secretion. In contrast, treatment with TGF-β reduced the migratory response and collagenase secretion. Since the multilayered structure is rich in collagenous matrix, degradation of the matrix by secreted collagenase is probably necessary for the cells to migrate into the wounded areas. Furthermore, malotilate, which is now under development as an agent for wound therapy, facilitated the migratory response of NHDF with increased collagenase secretion in this wound model, suggesting that the wound healing effect of malotilate is in part attributable to stimulated migration of fibroblasts to wounded areas subsequent to extracellular matrix-degradation.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalJournal of dermatological science
Volume17
Issue number2
DOIs
Publication statusPublished - 1998 Jun 1

Keywords

  • Cell- migration
  • Collagenase
  • Human dermal fibroblasts
  • Malotilate
  • Three-dimensional culture
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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