Fertilization cone formation in starfish oocytes: The role of the egg cortex actin microfilaments in sperm incorporation

Keiichiro Kyozuka, Kenzi Osanai

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The process of sperm incorporation into starfish (Asterias amurensis) oocytes was examined by electron and fluorescence microscopy. The fertilization cone began to form at the place where the acrosomal process fused with the egg surface and developed into an inverted conical mass containing a small amount of electron‐dense cytoplasm. Microfilaments, which stained with NBD‐phallacidin, were detected in the fertilization cone. Microvillar protrusions from the fully grown fertilization cone engulfed the sperm head outside the fertilization membrane. The sperm organelles were incorporated into the egg cortex with the absorption of the protrusions. Cytochalasin B inhibited sperm incorporation, fertilization cone formation, and actin filament organization. It is suggested that the development and reduction of the fertilization cone, which depend on the functioning of microfilaments, are necessary for sperm incorporation in starfish.

Original languageEnglish
Pages (from-to)275-285
Number of pages11
JournalGamete Research
Volume20
Issue number3
DOIs
Publication statusPublished - 1988 Jul

Keywords

  • Asterias amurensis
  • NBD‐phallacidin
  • acrosomal process
  • cytochalasin B
  • insemination
  • oocytes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'Fertilization cone formation in starfish oocytes: The role of the egg cortex actin microfilaments in sperm incorporation'. Together they form a unique fingerprint.

  • Cite this