Ferret Podoplanin Is Detected by PMab-241 in Immunohistochemistry

Ren Nanamiya, Junko Takei, Teizo Asano, Masato Sano, Tomohiro Tanaka, Hideki Hosono, Hiroyuki Harada, Yusuke Sakai, Takuya Mizuno, Hiroyoshi Suzuki, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

Abstract

Podoplanin (PDPN) plays an important role in the development of many normal tissues and is expressed in various cancers. We have previously developed multiple monoclonal antibodies (mAbs) against PDPNs from a variety of animal species and characterized each of these PDPNs using the anti-PDPN mAbs. In this study, we evaluated whether these anti-PDPN mAbs possess cross-reactivity with ferret PDPN (ferPDPN) using flow cytometry. Comprehensive analysis using 17 differing anti-PDPN mAbs available for immunohistochemistry use, demonstrated that the anti-bear PDPN mAb (clone PMab-241) strongly cross-reacts with ferPDPN-overexpressed Chinese hamster ovary-K1 (CHO/ferPDPN) cells. Immunohistochemistry analysis demonstrated intense PMab-241 staining within Bowman's capsules and glomeruli of the ferret kidney, and lymphatic endothelial cells of the ferret lung. These results demonstrate that PMab-241 is suitable for the detection of PDPN in ferret tissues.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume40
Issue number3
DOIs
Publication statusPublished - 2021 Jun
Externally publishedYes

Keywords

  • PDPN
  • ferret podoplanin
  • immunohistochemistry

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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