Femtosecond spectroscopy of acidified bacteriorhodopsin

Takayoshi Kobayashi, Mamoru Terauchi, Masayuki Yoshizawa, Makoto Taiji, Tsutomu Kouyama

Research output: Contribution to conferencePaper

Abstract

Bacteriorhodopsin (bR) is a light-harvesting energy-transducing pigment so far found only in Halobacterium halobium, prokaryotes that occur in natural brines. The acidic form of bR (bR605) is an approximately 40:60 mixture of 13-cis bR605 and all-trans bR605. A study of the primary photoprocesses of bR605 by femtosecond absorption spectroscopy is described. It has been found that the lowest excited singlet states of 13-cis and all-trans bR605 have different lifetimes (1.5 ± 0.2 ps for 13-cis bR605 and 8.6 ± 0.9 ps for all-trans bR605) and that the red-shifted ground state intermediate so far reported in the nanosecond time scale Kacid is formed directly from the lowest excited singlet states of bR605. It has also been found that the isomerization around C13 = C14 of the retinal molecule in the lowest excited singlet states in both 13-cis bR605 and all-trans bR605 has an overdamped nature, i.e., the isomerization angle varies only monotonously in the lowest excited singlet states of both 13-crs bR605 and all-trans bR605.

Original languageEnglish
Number of pages1
Publication statusPublished - 1990 Dec 1
Externally publishedYes
Event17th International Conference on Quantum Electronics - IQEC '90 - Anaheim, CA, USA
Duration: 1990 May 211990 May 25

Other

Other17th International Conference on Quantum Electronics - IQEC '90
CityAnaheim, CA, USA
Period90/5/2190/5/25

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Kobayashi, T., Terauchi, M., Yoshizawa, M., Taiji, M., & Kouyama, T. (1990). Femtosecond spectroscopy of acidified bacteriorhodopsin. Paper presented at 17th International Conference on Quantum Electronics - IQEC '90, Anaheim, CA, USA, .