Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis

Kiyoshi Mashiguchi, Eriko Sasaki, Yukihisa Shimada, Miyu Nagae, Kotomi Ueno, Takeshi Nakano, Koichi Yoneyama, Yoshihito Suzuki, Tadao Asami

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)

Abstract

Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling.

Original languageEnglish
Pages (from-to)2460-2465
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number11
DOIs
Publication statusPublished - 2009 Nov
Externally publishedYes

Keywords

  • Arabidopsis
  • Feedback regulation
  • Microarray
  • Shoot branching
  • Strigolactone

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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