Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis

Kiyoshi Mashiguchi, Eriko Sasaki, Yukihisa Shimada, Miyu Nagae, Kotomi Ueno, Takeshi Nakano, Koichi Yoneyama, Yoshihito Suzuki, Tadao Asami

    Research output: Contribution to journalArticle

    107 Citations (Scopus)

    Abstract

    Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling.

    Original languageEnglish
    Pages (from-to)2460-2465
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume73
    Issue number11
    DOIs
    Publication statusPublished - 2009 Nov 1

    Keywords

    • Arabidopsis
    • Feedback regulation
    • Microarray
    • Shoot branching
    • Strigolactone

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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  • Cite this

    Mashiguchi, K., Sasaki, E., Shimada, Y., Nagae, M., Ueno, K., Nakano, T., Yoneyama, K., Suzuki, Y., & Asami, T. (2009). Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis. Bioscience, Biotechnology and Biochemistry, 73(11), 2460-2465. https://doi.org/10.1271/bbb.90443