Feasibility study for functional test battery of SOD transgenic rat (H46R) and evaluation of edaravone, a free radical scavenger

Masashi Aoki, Hitoshi Warita, Hideki Mizuno, Naoki Suzuki, Satoshi Yuki, Yasuto Itoyama

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We evaluated a battery of functional tests for investigating the effects of edaravone, a free radical scavenger, in SOD1 transgenic (H46R) rat model of amyotrophic lateral sclerosis. Edaravone (1.5 or 3.0 mg/kg/h) or saline was administered intravenously to rats by continuous infusion (1 h per day) for 2 days, followed by a 2-day holiday. Lifetime and duration of illness were evaluated, and motor function was assessed using the hind-foot reflex test, landing foot-splay test, rota rod test and inclined plate test at a predetermined time point at which half of the control animals had died. Statistical comparison of motor functions of edaravone-treated and control SOD1 transgenic rats at an objectively determined time point was confirmed to be feasible. Edaravone-treated male rats showed significantly better performance in the landing foot-splay test. The present model seems suitable for evaluating motor function of H46R SOD1 transgenic rats, and be useful for examining the therapeutic potential of edaravone to treat amyotrophic lateral sclerosis.

Original languageEnglish
Pages (from-to)321-325
Number of pages5
JournalBrain research
Volume1382
DOIs
Publication statusPublished - 2011 Mar 25

Keywords

  • Amyotrophic lateral sclerosis
  • Free radical
  • SOD1
  • Transgenic rats

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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