TY - JOUR
T1 - Feasibility study for functional test battery of SOD transgenic rat (H46R) and evaluation of edaravone, a free radical scavenger
AU - Aoki, Masashi
AU - Warita, Hitoshi
AU - Mizuno, Hideki
AU - Suzuki, Naoki
AU - Yuki, Satoshi
AU - Itoyama, Yasuto
PY - 2011/3/25
Y1 - 2011/3/25
N2 - We evaluated a battery of functional tests for investigating the effects of edaravone, a free radical scavenger, in SOD1 transgenic (H46R) rat model of amyotrophic lateral sclerosis. Edaravone (1.5 or 3.0 mg/kg/h) or saline was administered intravenously to rats by continuous infusion (1 h per day) for 2 days, followed by a 2-day holiday. Lifetime and duration of illness were evaluated, and motor function was assessed using the hind-foot reflex test, landing foot-splay test, rota rod test and inclined plate test at a predetermined time point at which half of the control animals had died. Statistical comparison of motor functions of edaravone-treated and control SOD1 transgenic rats at an objectively determined time point was confirmed to be feasible. Edaravone-treated male rats showed significantly better performance in the landing foot-splay test. The present model seems suitable for evaluating motor function of H46R SOD1 transgenic rats, and be useful for examining the therapeutic potential of edaravone to treat amyotrophic lateral sclerosis.
AB - We evaluated a battery of functional tests for investigating the effects of edaravone, a free radical scavenger, in SOD1 transgenic (H46R) rat model of amyotrophic lateral sclerosis. Edaravone (1.5 or 3.0 mg/kg/h) or saline was administered intravenously to rats by continuous infusion (1 h per day) for 2 days, followed by a 2-day holiday. Lifetime and duration of illness were evaluated, and motor function was assessed using the hind-foot reflex test, landing foot-splay test, rota rod test and inclined plate test at a predetermined time point at which half of the control animals had died. Statistical comparison of motor functions of edaravone-treated and control SOD1 transgenic rats at an objectively determined time point was confirmed to be feasible. Edaravone-treated male rats showed significantly better performance in the landing foot-splay test. The present model seems suitable for evaluating motor function of H46R SOD1 transgenic rats, and be useful for examining the therapeutic potential of edaravone to treat amyotrophic lateral sclerosis.
KW - Amyotrophic lateral sclerosis
KW - Free radical
KW - SOD1
KW - Transgenic rats
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U2 - 10.1016/j.brainres.2011.01.058
DO - 10.1016/j.brainres.2011.01.058
M3 - Article
C2 - 21276427
AN - SCOPUS:79952537198
VL - 1382
SP - 321
EP - 325
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
ER -