Fatty acid production from a highly CO2 tolerant alga, Chlorocuccum littorale, in the presence of inorganic carbon and nitrate

Masaki Ota, Yoshitaka Kato, Hiromoto Watanabe, Masaru Watanabe, Yoshiyuki Sato, Richard L. Smith, Hiroshi Inomata

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Photoautotrophic fatty acid production of a highly CO2-tolerant green alga Chlorococcum littorale was investigated in the presence of inorganic carbon and nitrate at 295 K and a light intensity of 170 μmol-photon m-2 s-1. CO2 concentration in the bubbling gas was adjusted by mixing pure gas components of CO2 and N2 to avoid photorespiration and β-oxidation of fatty acids under O2 atmosphere conditions. Fatty acid content was almost constant for the CO2 concentrations ranging from 5% to 50% under nitrate-rich conditions corresponding to the logarithmic growth phase. After nitrate depletion, the content drastically increased with a decrease in CO2 concentration. HCO3- / CO2 ratio in the culture media was found to be a controlling factor for fatty acid production after the nitrate limitation phase. For a CO2 concentration of 5%, the fatty acid content was ca. 34 wt.% at maximum, which is comparable with other land plant seed oils.

Original languageEnglish
Pages (from-to)5237-5242
Number of pages6
JournalBioresource Technology
Volume100
Issue number21
DOIs
Publication statusPublished - 2009 Nov 1

Keywords

  • Algal oil
  • Biofuels
  • Biomass
  • CO
  • Microalgae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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