TY - JOUR
T1 - Fatty Acid Metabolism in Bacteria That Produce Eicosapentaenoic Acid Isolated from Sea Urchin Strongylocentrotus nudus
AU - Iwanami, Hiromitsu
AU - Yamaguchi, Toshiyasu
AU - Takeuchi, Masaaki
PY - 1995
Y1 - 1995
N2 - The biosynthetic pathway of 5Z, 8Z, 11 Z, 14Z, 18Z-eicosapentaenoic acid (EPA, C20: 5 n-3) in Vibrio sp. isolated from sea urchin was studied. EPA was one of the major fatty acid constituents (17.5%) in the bacteria, while the other polyunsaturated fatty acids were trace elements (<1.0%). The radioactivity in the fatty acids was measured by a liquid scintillation counter after separating with argentic impregnated thin layer chromatography. The conversion from [1-14C]C18: ln-9 to C20: 5n-3 was oxygen-dependent and cerulenin-sensitive. In the presence of cerulenin, the accumulation of radioactive trienoic fatty acid increased and tetra- and pentaenoic acids decreased. These results suggest that C18: ln-9 was aerobically desaturated and elongated to C20: 5n-3 via C18: 3. Since [1-14C] C20 : 4n-6 and [1-14C] C18 : 3n-3 were converted to C20 : 5n-3, the desaturation of n-6 to n-3 unsaturated fatty acids occur in both Cl8 and C20 chain length.
AB - The biosynthetic pathway of 5Z, 8Z, 11 Z, 14Z, 18Z-eicosapentaenoic acid (EPA, C20: 5 n-3) in Vibrio sp. isolated from sea urchin was studied. EPA was one of the major fatty acid constituents (17.5%) in the bacteria, while the other polyunsaturated fatty acids were trace elements (<1.0%). The radioactivity in the fatty acids was measured by a liquid scintillation counter after separating with argentic impregnated thin layer chromatography. The conversion from [1-14C]C18: ln-9 to C20: 5n-3 was oxygen-dependent and cerulenin-sensitive. In the presence of cerulenin, the accumulation of radioactive trienoic fatty acid increased and tetra- and pentaenoic acids decreased. These results suggest that C18: ln-9 was aerobically desaturated and elongated to C20: 5n-3 via C18: 3. Since [1-14C] C20 : 4n-6 and [1-14C] C18 : 3n-3 were converted to C20 : 5n-3, the desaturation of n-6 to n-3 unsaturated fatty acids occur in both Cl8 and C20 chain length.
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U2 - 10.2331/suisan.61.205
DO - 10.2331/suisan.61.205
M3 - Article
AN - SCOPUS:85008124114
VL - 61
SP - 205
EP - 210
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 2
ER -