Fatty acid-binding protein regulates LPS-induced TNF-α production in mast cells

Noriko Yamamoto, Izumi Kaneko, Keiju Motohashi, Hiroyuki Sakagami, Yasuhiro Adachi, Nobuko Tokuda, Tomoo Sawada, Hiroshi Furukawa, Yoshiya Ueyama, Kohji Fukunaga, Masao Ono, Hisatake Kondo, Yuji Owada

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

There has been increasing evidence for the involvement of fatty acid-binding proteins (FABPs) in the cytokine production of macrophages and dendritic cells probably through the control of cellular lipid metabolism and signal transduction. Since mast cells (MCs) are recently shown to be involved in immune response through modification of cytokine production, it is possible that some FABPs could also be involved in the immune response of MCs. In this study, we found that epidermal-type FABP (E-FABP) was expressed in murine bone marrow-derived MCs (BMMCs). Using BMMCs from genetically E-FABP-null mutated mice, we demonstrated that E-FABP in BMMCs plays a key role in the production of TNF-α following lipopolysaccharide (LPS) stimulation. In the in vivo septic peritonitis model (cecal ligation and puncture model), E-FABP-null mice showed a significantly increased mortality compared to wild-type mice. However, no significant difference in antigen-induced cytokine production was observed between wild-type and E-FABP-null BMMCs, and systemic anaphylaxis was equally induced in vivo in both wild-type and E-FABP-null mice. These results suggest that E-FABP is specifically involved in the LPS-induced cytokine production of MCs, and could play a role in the host-defense against bacterial infection, possibly through regulation of TNF-α production.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume79
Issue number1-2
DOIs
Publication statusPublished - 2008 Jul

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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