Fast microscopical dissection of action scenes played by Escherichia coli RNA polymerase

Yuki Suzuki, Minsang Shin, Aiko Yoshida, Shige H. Yoshimura, Kunio Takeyasu

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Using fast-scanning atomic force microscopy, we directly visualized the interaction of Escherichia coli RNA polymerase (RNAP) with DNA at the scan rate of 1-2 frames per second. The analyses showed that the RNAP can locate the promoter region not only by sliding but also by hopping and/or segmental transfer. Upon the addition of 0.05 mM NTPs to the stalled complex, the RNAP molecule pulled the template DNA uni-directionally at the rates of 15 nucleotides/s on average. The present method is potentially applicable to examine a variety of protein-nucleic acid interactions, especially those involved in the process of gene regulation.

Original languageEnglish
Pages (from-to)3187-3192
Number of pages6
JournalFEBS Letters
Volume586
Issue number19
DOIs
Publication statusPublished - 2012 Sep 21
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Fast-scanning atomic force microscopy
  • Protein-DNA interaction
  • RNA polymerase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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