Fast DNA interstrand cross-linking reaction by 6-vinylpurine

Shuhei Imoto, Tomoko Chikuni, Hisao Kansui, Takehisa Kunieda, Fumi Nagatsugi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Oligonucleotides that incorporate a reactive moiety to form an interstrand cross-link have been widely studied for their potential toward inhibiting gene expression or as basic tools for chemical biology studies. The 6-vinylpurine (2) newly designed in the current study serves well as a new purine-base moiety for increasing cross-link reactivity to target cytosine. Thus, oligonucleotides containing 6-vinylpurine exhibit a more selective and much smoother DNA cross-linking ability to cytosine than the oligonucleotide analogs derived from 2-amino-6-vinylpurine (1) previously explored.

Original languageEnglish
Pages (from-to)752-762
Number of pages11
JournalNucleosides, Nucleotides and Nucleic Acids
Volume31
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

Keywords

  • DNA interstrand cross-link
  • Oligonucleotide
  • Purine analogs

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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