Fas-mediated apoptosis is enhanced by glycyrrhizin without alteration of caspase-3-like activity

Shunji Ishiwata, Kei Nakashita, Yasuko Ozawa, Masahiro Niizeki, Seishiro Nozaki, Yoshihisa Tomioka, Michinao Mizugaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We demonstrate that glycyrrhizin (GL) enhanced Fas-mediated apoptotic body formation and DNA fragmentation in T cell lines although GL alone did not induce apoptosis. The enhancement effect of Fas-mediated apoptosis by GL was dose-dependent above 0.3 μM. Time course study revealed that simultaneous co-treatment of GL and anti-Fas antibody was crucial for the enhancement of apoptosis and pretreatment with GL was not effective. Anti-Fas antibody elicited caspase-3-like activity. However caspase-3-like activity with co-treatment of GL and anti-Fas antibody was the same level as the antibody alone. Glycyrrhetic acid, the aglycon of GL, did not enhance Fas- mediated apoptosis. The amphipathic property of GL might enable it to interact with the plasma membrane and lead to the enhancement of apoptosis.

Original languageEnglish
Pages (from-to)1163-1166
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number11
DOIs
Publication statusPublished - 1999 Nov

Keywords

  • Apoptosis
  • Caspase-3
  • Fas
  • Glycyrrhetic acid
  • Glycyrrhizin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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