Factors that enhance Escherichia coli-expressed TRβ binding to T3 and DNA

P. M. Yen, A. Sugawara, Y. Liu, J. Whang, W. W. Chin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Thyroid hormone receptors (TRs) recently have been produced in E. coli by several laboratories. We produced E. coli-expressed human TRβ using the histidine/fusion protein system. Surprisingly, we observed that reticulocyte lysate, nonspecific proteins, and 1% Triton X dramatically increased both the T3- and DNA-binding activities of human TRβ. These studies demonstrate that there are a number of factors that will enhance ligand and DNA binding of E. coli-expressed TRβ. Addition of these factors to reaction samples containing E. coli-expressed TRs will help to optimize measurement conditions. These findings also suggest that experiments in which cellular proteins are added to highly purified TR preparations may require controls to eliminate contributions by nonspecific proteins.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalThyroid
Volume5
Issue number4
DOIs
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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