TY - JOUR
T1 - Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar
AU - Ino, Kosuke
AU - Fukuda, Mika T.
AU - Hiramoto, Kaoru
AU - Taira, Noriko
AU - Nashimoto, Yuji
AU - Shiku, Hitoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (A) (No. 16H02280 ) and a Grant-in-Aid for Scientific Research (B) (Nos. 18H01840 , and 18H01999 ) from the Japan Society for the Promotion of Science . This work was also supported by Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University . This work was also supported by the Shimadzu Science Foundation , the Nakatani Foundation , and the Kato Foundation for Promotion of Science .
Publisher Copyright:
© 2020 The Society for Biotechnology, Japan
PY - 2020/11
Y1 - 2020/11
N2 - Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca2+ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl2 solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications.
AB - Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca2+ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl2 solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications.
KW - 3D hydrogel
KW - Calcium alginate hydrogel
KW - Cell culture
KW - Molten sugar
KW - Sacrificial template of sugar
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U2 - 10.1016/j.jbiosc.2020.06.014
DO - 10.1016/j.jbiosc.2020.06.014
M3 - Article
C2 - 32758401
AN - SCOPUS:85089006406
VL - 130
SP - 539
EP - 544
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -