Fabrication of multiphenotype cell arrays within reversibly sealed microfluidic channels for high-throughput analysis

Ali Khademhosseini, George Eng, Judy Yeh, Jeffrey Karp, Hirokazu Kaji, Jeffrey Borenstein, Omid Farokhzad, Robert Langer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a soft lithographic method to fabricate multiphenotype cell arrays by capturing cells within an array of reversibly sealed microfluidic channels made of elastomeric polydimethylsiloxane (PDMS) molds. Upon formation of the cell arrays on the substrate, the PDMS mold could be removed, and the orthogonal alignment and subsequent attachment of a secondary array of channels on the patterned substrates could be used to sequentially deliver fluids to the patterned cells. The ability to position many cell types on particular regions within a two-dimensional substrate could potentially lead to improved high-throughput methods applicable to drug screening and tissue engineering.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages886-888
Number of pages3
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005 Jan 1
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 2005 Oct 92005 Oct 13

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period05/10/905/10/13

Keywords

  • Cellular arrays
  • Microfluidics
  • Multi-phenotype
  • PDMS

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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