Fabrication of cell-adhesion surface and capillary vessel model by photolithography

Takuma Nakano, Mika Tada, Yu Ching Lin, Seiichi Ikeda, Tomoyuki Uchida, Hiroyuki Oura, Toshio Fukuda, Takehisa Matsuda, Makoto Negoro, Fumihito Arai

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    5 Citations (Scopus)

    Abstract

    We have been developing scaffolds of three-dimensional (3D) synthetic vascular prosthesis in tailor-made. Human umbilical vein endothelial cells (HUVECs) attached on the inner surface of the scaffold have anticoagulant effects. Asperity structures of the inner surface are important to cell adhesion. It is important to quantify the inner surface asperity condition of the scaffold by observing HUVECs behavior and morphology. For this purpose, we recreated the inner surface profile of the scaffold on a poly(dimethilsiloxane) (PDMS) substrate by microfabrication. We made semiround convex patterns of resist that had 8 μm in diameter and 5 μm high using photolithography, and the concave pattern on the PDMS substrate by printing. We observed HUVECs adhering to the PDMS substrate having concave pattern on it surface. The distribution density of the concaves of the tested pattern is 1600 /mm 2 or 40,000 in a 25 mm2 area. In addition, we fabricated a capillary vessel model by photolithography, creating a branched capillary tube model that had 13 gm in diameter. We confirmed that the capillary vessel model had no leakage using a methylene blue solution flow in the channel.

    Original languageEnglish
    Title of host publication2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
    Pages350-355
    Number of pages6
    DOIs
    Publication statusPublished - 2007 Dec 1
    Event2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS - Nagoya, Japan
    Duration: 2007 Nov 112007 Nov 14

    Publication series

    Name2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS

    Other

    Other2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
    CountryJapan
    CityNagoya
    Period07/11/1107/11/14

    Keywords

    • Biology
    • Blood vessel
    • Endothelial cell
    • Photolithography
    • Regenerative medicine

    ASJC Scopus subject areas

    • Artificial Intelligence
    • Computer Networks and Communications
    • Human-Computer Interaction
    • Electrical and Electronic Engineering

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  • Cite this

    Nakano, T., Tada, M., Lin, Y. C., Ikeda, S., Uchida, T., Oura, H., Fukuda, T., Matsuda, T., Negoro, M., & Arai, F. (2007). Fabrication of cell-adhesion surface and capillary vessel model by photolithography. In 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS (pp. 350-355). [4420879] (2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS). https://doi.org/10.1109/MHS.2007.4420879