TY - GEN
T1 - Fabrication of cell-adhesion surface and capillary vessel model by photolithography
AU - Nakano, Takuma
AU - Tada, Mika
AU - Lin, Yu Ching
AU - Ikeda, Seiichi
AU - Uchida, Tomoyuki
AU - Oura, Hiroyuki
AU - Fukuda, Toshio
AU - Matsuda, Takehisa
AU - Negoro, Makoto
AU - Arai, Fumihito
PY - 2007
Y1 - 2007
N2 - We have been developing scaffolds of three-dimensional (3D) synthetic vascular prosthesis in tailor-made. Human umbilical vein endothelial cells (HUVECs) attached on the inner surface of the scaffold have anticoagulant effects. Asperity structures of the inner surface are important to cell adhesion. It is important to quantify the inner surface asperity condition of the scaffold by observing HUVECs behavior and morphology. For this purpose, we recreated the inner surface profile of the scaffold on a poly(dimethilsiloxane) (PDMS) substrate by microfabrication. We made semiround convex patterns of resist that had 8 μm in diameter and 5 μm high using photolithography, and the concave pattern on the PDMS substrate by printing. We observed HUVECs adhering to the PDMS substrate having concave pattern on it surface. The distribution density of the concaves of the tested pattern is 1600 /mm 2 or 40,000 in a 25 mm2 area. In addition, we fabricated a capillary vessel model by photolithography, creating a branched capillary tube model that had 13 gm in diameter. We confirmed that the capillary vessel model had no leakage using a methylene blue solution flow in the channel.
AB - We have been developing scaffolds of three-dimensional (3D) synthetic vascular prosthesis in tailor-made. Human umbilical vein endothelial cells (HUVECs) attached on the inner surface of the scaffold have anticoagulant effects. Asperity structures of the inner surface are important to cell adhesion. It is important to quantify the inner surface asperity condition of the scaffold by observing HUVECs behavior and morphology. For this purpose, we recreated the inner surface profile of the scaffold on a poly(dimethilsiloxane) (PDMS) substrate by microfabrication. We made semiround convex patterns of resist that had 8 μm in diameter and 5 μm high using photolithography, and the concave pattern on the PDMS substrate by printing. We observed HUVECs adhering to the PDMS substrate having concave pattern on it surface. The distribution density of the concaves of the tested pattern is 1600 /mm 2 or 40,000 in a 25 mm2 area. In addition, we fabricated a capillary vessel model by photolithography, creating a branched capillary tube model that had 13 gm in diameter. We confirmed that the capillary vessel model had no leakage using a methylene blue solution flow in the channel.
KW - Biology
KW - Blood vessel
KW - Endothelial cell
KW - Photolithography
KW - Regenerative medicine
UR - http://www.scopus.com/inward/record.url?scp=50149110071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50149110071&partnerID=8YFLogxK
U2 - 10.1109/MHS.2007.4420879
DO - 10.1109/MHS.2007.4420879
M3 - Conference contribution
AN - SCOPUS:50149110071
SN - 9781424418589
T3 - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
SP - 350
EP - 355
BT - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
T2 - 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
Y2 - 11 November 2007 through 14 November 2007
ER -