Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles

Mayu Tayama, Tomoya Inose, Noriko Yamauchi, Kouichi Nakashima, Masayuki Tokunaga, Chihiro Kato, Kohsuke Gonda, Yoshio Kobayashi

Research output: Contribution to journalArticlepeer-review

Abstract

This work proposed a method for fabricating poly(lactic-co-glycolic acid) (PLGA) nanoparticles (ca. 70 nm) loaded with indocyanine green (ICG) molecules by an antisolvent crystallization method using dimethyl sulfoxide (DMSO) as a good solvent and water as a poor solvent. ICG molecules were loaded to PLGA nanoparticles by mixing PLGA, polyethylenimine, and polyethylene glycol (PEG) in DMSO with polyvinyl alcohol, albumin, and ICG in water. The ICG-loaded PLGA nanoparticle colloid solutions emitted fluorescence even at high ICG concentrations. Not only the tissues of a tumor-bearing mouse but also the tumor could be imaged with an IVIS system by injecting ICG-loaded PLGA nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity, though some ICG-loaded PLGA nanoparticles were trapped in tissues such as the liver and spleen due to insufficient PEGylation. Thus, this work showed that ICG-loaded PLGA nanoparticles have potential for cancer imaging. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
JournalColloid and Polymer Science
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • Antisolvent crystallization
  • Indocyanine green fluorescent marker
  • Nanoparticles
  • Poly (lactic-co-glycolic acid)

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Colloid and Surface Chemistry
  • Materials Chemistry

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