TY - JOUR
T1 - Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering
AU - Fujioka-Kobayashi, Masako
AU - Tsuru, Kanji
AU - Nagai, Hirokazu
AU - Fujisawa, Kenji
AU - Kudoh, Takaharu
AU - Ohe, Go
AU - Ishikawa, Kunio
AU - Miyamoto, Youji
PY - 2018/10
Y1 - 2018/10
N2 - Carbonate apatite-coated calcium carbonate (CO3Ap/CaCO3) was fabricated through a dissolution–precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3, 10% CO3Ap/CaCO3, 30% CO3Ap/CaCO3, and CO3Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3Ap. Interestingly, in a rabbit femur defect model, 10% CO3Ap/CaCO3 induced significantly higher new bone formation and higher material resorption compared with CO3Ap at 8 weeks. Nevertheless, CO3Ap showed a superior osteoconductive potential compared with 10% CO3Ap/CaCO3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO3Ap/CaCO3 has a positive potential for bone tissue engineering based on well-controlled calcium release, bone formation, and material resorption.
AB - Carbonate apatite-coated calcium carbonate (CO3Ap/CaCO3) was fabricated through a dissolution–precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3, 10% CO3Ap/CaCO3, 30% CO3Ap/CaCO3, and CO3Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3Ap. Interestingly, in a rabbit femur defect model, 10% CO3Ap/CaCO3 induced significantly higher new bone formation and higher material resorption compared with CO3Ap at 8 weeks. Nevertheless, CO3Ap showed a superior osteoconductive potential compared with 10% CO3Ap/CaCO3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO3Ap/CaCO3 has a positive potential for bone tissue engineering based on well-controlled calcium release, bone formation, and material resorption.
KW - bone regeneration
KW - bone substitute
KW - bone tissue engineering
KW - calcium carbonate
KW - carbonate apatite
KW - osteoconductivity
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U2 - 10.1002/term.2742
DO - 10.1002/term.2742
M3 - Article
C2 - 30058260
AN - SCOPUS:85052842255
VL - 12
SP - 2077
EP - 2087
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 10
ER -