Fabricating of acetylcholine biosensor by a layer-by-layer deposition technique for determining trichlorfon

Haibin Shi, Zixia Zhao, Zhao Song, Jiadong Huang, Yu Yang, Junichi Anzai, Tetsuo Osa, Qiang Chen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

An acetylcholine (ACh) biosensor has been fabricated with bienzymes/poly(diallyldimethylammonium chloride) (PDDA) multilayer film-modified platinum (Pt) electrodes by a layer-by-layer technique (LBL). The ACh biosensor was optimized and the properties are described. This ACh biosensor was used for the detection of organophosphate pesticide trichlorfon. The detection limits (found 0.001 μg/mL for trichlorfon) make it possible to detect the pollutants. This simple protocol of biosensor preparation, high sensitivity and stability are very promising for the determination of environmental pollutants in field conditions.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
JournalElectroanalysis
Volume17
Issue number14
DOIs
Publication statusPublished - 2005 Jul 1

Keywords

  • Acetylcholinesterase
  • Biosensor
  • Layer-by-layer
  • PDDA
  • Trichlorfon

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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