FABP7 expression in normal and stab-injured brain cortex and its role in astrocyte proliferation

Kazem Sharifi, Yusuke Morihiro, Motoko Maekawa, Yuki Yasumoto, Hisae Hoshi, Yasuhiro Adachi, Tomoo Sawada, Nobuko Tokuda, Hisatake Kondo, Takeo Yoshikawa, Michiyasu Suzuki, Yuji Owada

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Reactive gliosis, in which astrocytes as well as other types of glial cells undergo massive proliferation, is a common hallmark of all brain pathologies. Brain-type fatty acid-binding protein (FABP7) is abundantly expressed in neural stem cells and astrocytes of developing brain, suggesting its role in differentiation and/or proliferation of glial cells through regulation of lipid metabolism and/or signaling. However, the role of FABP7 in proliferation of glial cells during reactive gliosis is unknown. In this study, we examined the expression of FABP7 in mouse cortical stab injury model and also the phenotype of FABP7-KO mice in glial cell proliferation. Western blotting showed that FABP7 expression was increased significantly in the injured cortex compared with the contralateral side. By immunohistochemistry, FABP7 was localized to GFAP + astrocytes (21% of FABP7 + cells) and NG 2+ oligodendrocyte progenitor cells (62%) in the normal cortex. In the injured cortex there was no change in the population of FABP7 +/NG2 + cells, while there was a significant increase in FABP7 +/GFAP + cells. In the stab-injured cortex of FABP7-KO mice there was decrease in the total number of reactive astrocytes and in the number of BrdU? astrocytes compared with wild-type mice. Primary cultured astrocytes from FABP7-KO mice also showed a significant decrease in proliferation and omega-3 fatty acid incorporation compared with wild-type astrocytes. Overall, these data suggest that FABP7 is involved in the proliferation of astrocytes by controlling cellular fatty acid homeostasis.

Original languageEnglish
Pages (from-to)501-513
Number of pages13
JournalHistochemistry and Cell Biology
Volume136
Issue number5
DOIs
Publication statusPublished - 2011 Nov

Keywords

  • Astrocyte
  • Brain injury
  • FABP7
  • Oligodendrocyte progenitor cell
  • Reactive gliosis

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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