Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

Shigeki Kato; Yasuaki Mohri; Tsuyoshi Matsuo; Eisaku Ogawa; Akihiro Umezawa; Ryuhei Okuyama; Katsuhiko Nishimori

doi:10.1016/j.febslet.2007.08.064

Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

Shigeki Kato, Yasuaki Mohri, Tsuyoshi Matsuo, Eisaku Ogawa, Akihiro Umezawa, Ryuhei Okuyama, Katsuhiko Nishimori

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.

Original language	English
Pages (from-to)	4685-4690
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2007.08.064
Publication status	Published - 2007 Oct 2

Keywords

EOB
GPCR
GPR48
Gene deletion mice
Keratinocyte
LGR4

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice",

abstract = "We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.",

keywords = "EOB, GPCR, GPR48, Gene deletion mice, Keratinocyte, LGR4",

author = "Shigeki Kato and Yasuaki Mohri and Tsuyoshi Matsuo and Eisaku Ogawa and Akihiro Umezawa and Ryuhei Okuyama and Katsuhiko Nishimori",

note = "Funding Information: We would like to thank Dr. J. Takeda for providing us the K5 Cre TG mice, and Dr. M. Kitagawa for valuable discussion. This research was supported in part by a Grants-in-Aid for Exploratory Research (#17659251) from the Ministry of education, Culture, Sports, Science and Technology of Japan, and by a donation from Kureha Corporation. ",

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AU - Kato, Shigeki

AU - Mohri, Yasuaki

AU - Matsuo, Tsuyoshi

AU - Ogawa, Eisaku

AU - Umezawa, Akihiro

AU - Okuyama, Ryuhei

AU - Nishimori, Katsuhiko

N1 - Funding Information: We would like to thank Dr. J. Takeda for providing us the K5 Cre TG mice, and Dr. M. Kitagawa for valuable discussion. This research was supported in part by a Grants-in-Aid for Exploratory Research (#17659251) from the Ministry of education, Culture, Sports, Science and Technology of Japan, and by a donation from Kureha Corporation.

PY - 2007/10/2

Y1 - 2007/10/2

N2 - We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.

AB - We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.

KW - EOB

KW - GPCR

KW - GPR48

KW - Gene deletion mice

KW - Keratinocyte

KW - LGR4

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ER -

Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

Abstract

Keywords

ASJC Scopus subject areas

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