Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

Shigeki Kato, Yasuaki Mohri, Tsuyoshi Matsuo, Eisaku Ogawa, Akihiro Umezawa, Ryuhei Okuyama, Katsuhiko Nishimori

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.

Original languageEnglish
Pages (from-to)4685-4690
Number of pages6
JournalFEBS Letters
Volume581
Issue number24
DOIs
Publication statusPublished - 2007 Oct 2

Keywords

  • EOB
  • GPCR
  • GPR48
  • Gene deletion mice
  • Keratinocyte
  • LGR4

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kato, S., Mohri, Y., Matsuo, T., Ogawa, E., Umezawa, A., Okuyama, R., & Nishimori, K. (2007). Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice. FEBS Letters, 581(24), 4685-4690. https://doi.org/10.1016/j.febslet.2007.08.064