Extrusion and removal of lipid from the cytoplasm of porcine oocytes at the germinal vesicle stage: Centrifugation under hypertonic conditions influences vitrification

Kenshiro Hara, Yasuyuki Abe, Nobuyuki Kumada, Nobuya Aono, Jin Kobayashi, Hiromichi Matsumoto, Hiroshi Sasada, Eimei Sato

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

In the present study, we examined a novel lipid removal method, centrifugation in solutions made hypertonic by adding 0.27 M sugar. This allowed the lipid to be extruded and removed without the loss of active mitochondria or extra cytoplasm. The type of sugar influenced the proportion of oocytes that could be stratified by centrifugation. Glucose induced the highest extrusion rate of lipid droplets. After vitrification the rates of survival, germinal vesicle breakdown and metaphase II were 30, 26, and 7%, respectively, for lipid-removed GV oocytes; this was significantly higher (P < 0.05) than for corresponding vitrified lipid-intact oocytes (2, 0, and 0%, respectively). These results indicated that this method is useful to remove whole lipid droplets without losing mitochondria and improves cryotolerance of porcine GV oocytes.

Original languageEnglish
Pages (from-to)216-222
Number of pages7
JournalCryobiology
Volume50
Issue number2
DOIs
Publication statusPublished - 2005 Apr

Keywords

  • Centrifugation
  • GV oocytes
  • Hypertonic condition
  • Lipid droplets
  • Pig
  • Vitrification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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