Extrinsic plasmalogens suppress neuronal apoptosis in mouse neuroblastoma Neuro-2A cells: Importance of plasmalogen molecular species

Shinji Yamashita, Susumu Kanno, Kiyotaka Nakagawa, Mikio Kinoshita, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The level of ethanolamine plasmalogen (Pls), a phospholipid with antioxidant activity, is known to decrease in the brains of patients suffering from Alzheimer's disease (AD). The present study was performed to determine the mechanism underlying the neuroprotective effect of Pls. Neuronal apoptosis induced by serum starvation was suppressed by Pls in a concentration-dependent manner. Pls also induced the suppression of p53 and cytochrome c expression, which was increased due to serum starvation. Further, the activation of apoptosis signal-regulating kinase 1 (ASK 1) was also inhibited by Pls. Among the phospholipids analyzed, only Pls displayed an antiapoptotic effect. Pls also inhibited the reduction of cellular phospholipid and 20:4 levels. These functions underlying the action of Pls may be responsible for the regulation of membrane functions and second messenger production. In addition, of the species investigated, Pls with 22:6 (DHA) displayed the strongest suppression of neuronal apoptosis; however, no significant differences in reactive oxygen species (ROS) scavenging activity was observed between various species of Pls. Overall, our results show the potential of Pls, especially those containing DHA, in the prevention of AD.

Original languageEnglish
Pages (from-to)61012-61020
Number of pages9
JournalRSC Advances
Volume5
Issue number75
DOIs
Publication statusPublished - 2015 Jan 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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